anti cd4 alexa fluor 647 (Bio-Rad)
Structured Review
Anti Cd4 Alexa Fluor 647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bovine+cd4/Mouse+anti+Bovine+CD4/pm41271900-321-17-21
Average 94 stars, based on 139 article reviews
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Bioprocessing:Article Title: Characterization of bovine lymphocytes stimulated in vitro by Dictyocaulus viviparus homogenate. Article Snippet: Adult Dictyocaulus viviparus homogenate induced proliferation of lymphocytes from naïve cattle.. We characterized the responding cells by carboxyfluorescein diacetate succinimidyl ester (CFSE) loading, for detection of proliferation, and antibody labelling for cell surface molecules.. Lymphocytes expressing CD4, CD8 and γ / δ TCR, rather than Ig expressing cells, proliferated after in vitro stimulation with D. viviparus homogenate. Article Title: Immunogenicity and Protection against Mycobacterium caprae Challenge in Goats Vaccinated with BCG and Revaccinated after One Year Article Snippet: Cells were incubated at 37 °C with 5% CO 2 , 95% humidity for 6 h. Then, brefeldin A (Sigma-Aldrich, Steinheim, Germany) was added at a final concentration of 10 μg/mL, and cells were incubated for a further 16 h. Stimulated PBMC were counted, and 3 × 10 5 cells/well were dispensed in 96-well plates. .. Cells were stained with mouse monoclonal antibodies (mAb) CC8 (IgG2A, conjugated to FITC), which recognizes Staining:Article Title: Immunogenicity and Protection against Mycobacterium caprae Challenge in Goats Vaccinated with BCG and Revaccinated after One Year Article Snippet: Cells were incubated at 37 °C with 5% CO 2 , 95% humidity for 6 h. Then, brefeldin A (Sigma-Aldrich, Steinheim, Germany) was added at a final concentration of 10 μg/mL, and cells were incubated for a further 16 h. Stimulated PBMC were counted, and 3 × 10 5 cells/well were dispensed in 96-well plates. .. Cells were stained with mouse monoclonal antibodies (mAb) CC8 (IgG2A, conjugated to FITC), which recognizes Incubation:Article Title: Experimental aerosol Mycobacterium bovis model of infection in goats. Article Snippet: The use of animal models is essential in testing the efficacy for novel therapies against tuberculosis (TB).. Calves and non-human primates are examples of large animal models currently used to test TB vaccine efficacy but these animals are difficult and very expensive to house under high containment conditions.. The goat may represent an effective but less expensive alternative for testing prototype vaccines against TB. |
