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anti cd4 alexa fluor 647  (Bio-Rad)


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    Structured Review

    Bio-Rad anti cd4 alexa fluor 647
    Anti Cd4 Alexa Fluor 647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bovine+cd4/Mouse+anti+Bovine+CD4/pm41271900-321-17-21
    Average 94 stars, based on 139 article reviews
    anti cd4 alexa fluor 647 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Characterization of bovine lymphocytes stimulated in vitro by Dictyocaulus viviparus homogenate.
    Article Snippet: Adult Dictyocaulus viviparus homogenate induced proliferation of lymphocytes from naïve cattle.. We characterized the responding cells by carboxyfluorescein diacetate succinimidyl ester (CFSE) loading, for detection of proliferation, and antibody labelling for cell surface molecules.. Lymphocytes expressing CD4, CD8 and γ / δ TCR, rather than Ig expressing cells, proliferated after in vitro stimulation with D. viviparus homogenate.

    Article Title: Immunogenicity and Protection against Mycobacterium caprae Challenge in Goats Vaccinated with BCG and Revaccinated after One Year
    Article Snippet: Cells were incubated at 37 °C with 5% CO 2 , 95% humidity for 6 h. Then, brefeldin A (Sigma-Aldrich, Steinheim, Germany) was added at a final concentration of 10 μg/mL, and cells were incubated for a further 16 h. Stimulated PBMC were counted, and 3 × 10 5 cells/well were dispensed in 96-well plates. .. Cells were stained with mouse monoclonal antibodies (mAb) CC8 (IgG2A, conjugated to FITC), which recognizes bovine CD4, and mAb IL-A116 (IgG3, conjugated to RPE), which recognizes bovine CD45RO (both from Bio-Rad Laboratories Inc., Hercules, CA, USA). .. CD4 and CD45RO stained cells were then fixed and permeabilized with Leucoperm TM (Bio-Rad), according to the manufacturer’s instructions, and incubated with mAb CC302 (IgG1, conjugated to Alexa Fluor ® 647, Bio-Rad), which recognizes bovine IFN-γ, for 30 min at room temperature.

    Staining:

    Article Title: Immunogenicity and Protection against Mycobacterium caprae Challenge in Goats Vaccinated with BCG and Revaccinated after One Year
    Article Snippet: Cells were incubated at 37 °C with 5% CO 2 , 95% humidity for 6 h. Then, brefeldin A (Sigma-Aldrich, Steinheim, Germany) was added at a final concentration of 10 μg/mL, and cells were incubated for a further 16 h. Stimulated PBMC were counted, and 3 × 10 5 cells/well were dispensed in 96-well plates. .. Cells were stained with mouse monoclonal antibodies (mAb) CC8 (IgG2A, conjugated to FITC), which recognizes bovine CD4, and mAb IL-A116 (IgG3, conjugated to RPE), which recognizes bovine CD45RO (both from Bio-Rad Laboratories Inc., Hercules, CA, USA). .. CD4 and CD45RO stained cells were then fixed and permeabilized with Leucoperm TM (Bio-Rad), according to the manufacturer’s instructions, and incubated with mAb CC302 (IgG1, conjugated to Alexa Fluor ® 647, Bio-Rad), which recognizes bovine IFN-γ, for 30 min at room temperature.

    Incubation:

    Article Title: Experimental aerosol Mycobacterium bovis model of infection in goats.
    Article Snippet: The use of animal models is essential in testing the efficacy for novel therapies against tuberculosis (TB).. Calves and non-human primates are examples of large animal models currently used to test TB vaccine efficacy but these animals are difficult and very expensive to house under high containment conditions.. The goat may represent an effective but less expensive alternative for testing prototype vaccines against TB.



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    Proliferation of <t>CD4</t> + T cells from RB51 vaccinated dairy-beef heifers in response to in vitro RB51 and PPDb stimulation. Mean numbers (gray bars) of CD4 + T cells proliferating in response to media only (no stimulation, white circles, n = 8), γ-irradiated RB51 (red circles, n = 8), and PPDb (blue circles, n = 8). *, RB51 or PPDb stimulated CD4 + T cells compared to unstimulated CD4 + T cells at respective time points. #, PPDb stimulated CD4 + T cells compared to RB51 stimulated CD4 + T cells at respective time points. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. *, #p ≤ 0.05; **p ≤ 0.01; ***, ###p ≤ 0.001 .
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    Proliferation of <t>CD4</t> + T cells from RB51 vaccinated dairy-beef heifers in response to in vitro RB51 and PPDb stimulation. Mean numbers (gray bars) of CD4 + T cells proliferating in response to media only (no stimulation, white circles, n = 8), γ-irradiated RB51 (red circles, n = 8), and PPDb (blue circles, n = 8). *, RB51 or PPDb stimulated CD4 + T cells compared to unstimulated CD4 + T cells at respective time points. #, PPDb stimulated CD4 + T cells compared to RB51 stimulated CD4 + T cells at respective time points. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. *, #p ≤ 0.05; **p ≤ 0.01; ***, ###p ≤ 0.001 .
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    Image Search Results


    Proliferation of CD4 + T cells from RB51 vaccinated dairy-beef heifers in response to in vitro RB51 and PPDb stimulation. Mean numbers (gray bars) of CD4 + T cells proliferating in response to media only (no stimulation, white circles, n = 8), γ-irradiated RB51 (red circles, n = 8), and PPDb (blue circles, n = 8). *, RB51 or PPDb stimulated CD4 + T cells compared to unstimulated CD4 + T cells at respective time points. #, PPDb stimulated CD4 + T cells compared to RB51 stimulated CD4 + T cells at respective time points. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. *, #p ≤ 0.05; **p ≤ 0.01; ***, ###p ≤ 0.001 .

    Journal: Frontiers in Immunology

    Article Title: Effect of co-vaccination of cattle with RB51 and BCG on vaccine-specific CD4 + T cell responses

    doi: 10.3389/fimmu.2025.1664398

    Figure Lengend Snippet: Proliferation of CD4 + T cells from RB51 vaccinated dairy-beef heifers in response to in vitro RB51 and PPDb stimulation. Mean numbers (gray bars) of CD4 + T cells proliferating in response to media only (no stimulation, white circles, n = 8), γ-irradiated RB51 (red circles, n = 8), and PPDb (blue circles, n = 8). *, RB51 or PPDb stimulated CD4 + T cells compared to unstimulated CD4 + T cells at respective time points. #, PPDb stimulated CD4 + T cells compared to RB51 stimulated CD4 + T cells at respective time points. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. *, #p ≤ 0.05; **p ≤ 0.01; ***, ###p ≤ 0.001 .

    Article Snippet: Cells were then incubated with FITC labeled anti-bovine CD4 (clone CC8, Cat. No. MCA1653F, BioRad, Hercules, CA) antibody for 15 min at RT.

    Techniques: In Vitro, Irradiation

    Proliferative CD4 + T cell responses in combo vaccinated dairy-beef heifers compared to RB51 or BCG vaccinates. (A) Mean numbers of RB51-specific CD4 + T cells (gray bars) in combo vaccinated heifers (black circles, n = 8) compared to RB51 vaccinated heifers (red circles, n = 8). Proliferation in unstimulated (white circles, n = 16) CD4 + T cells from both RB51 and combo vaccinated heifers was used as a control. (B) Mean numbers (gray bars) of BCG-specific CD4 + T cells in combo vaccinated heifers (black circles, n = 8) compared to BCG vaccinated heifers (blue circles, n = 8). Proliferation in unstimulated (white circles, n = 16) CD4 + T cells from both BCG and combo vaccinated heifers was used as a control. *, proliferating CD4 + T cells from combo vaccinated heifers compared to single vaccinated heifers at respective time points. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. NS, no significance. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 .

    Journal: Frontiers in Immunology

    Article Title: Effect of co-vaccination of cattle with RB51 and BCG on vaccine-specific CD4 + T cell responses

    doi: 10.3389/fimmu.2025.1664398

    Figure Lengend Snippet: Proliferative CD4 + T cell responses in combo vaccinated dairy-beef heifers compared to RB51 or BCG vaccinates. (A) Mean numbers of RB51-specific CD4 + T cells (gray bars) in combo vaccinated heifers (black circles, n = 8) compared to RB51 vaccinated heifers (red circles, n = 8). Proliferation in unstimulated (white circles, n = 16) CD4 + T cells from both RB51 and combo vaccinated heifers was used as a control. (B) Mean numbers (gray bars) of BCG-specific CD4 + T cells in combo vaccinated heifers (black circles, n = 8) compared to BCG vaccinated heifers (blue circles, n = 8). Proliferation in unstimulated (white circles, n = 16) CD4 + T cells from both BCG and combo vaccinated heifers was used as a control. *, proliferating CD4 + T cells from combo vaccinated heifers compared to single vaccinated heifers at respective time points. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. NS, no significance. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 .

    Article Snippet: Cells were then incubated with FITC labeled anti-bovine CD4 (clone CC8, Cat. No. MCA1653F, BioRad, Hercules, CA) antibody for 15 min at RT.

    Techniques: Control

    Th1 functional potential of vaccine-specific CD4 + T cells in dairy-beef heifers. (A) Quadrant gate defines functional subsets of CD4 + T cells: Q1, proliferating and IFN-γ + ; Q2, IFN-γ + only; Q3, unresponsive; Q4, proliferating only. (B, C) Bar height represents total average number of responding CD4 + T cells; broken down into IFN-γ + only (white bars), proliferating only (gray bars), and proliferating and IFN-γ + (black bars) subsets. (B) Mean number of RB51-specific CD4 + T cells exhibiting Th1 functionality in RB51 vaccinated (n = 8) and combo vaccinated (n = 8) heifers. (C) Mean number of BCG-specific CD4 + T cells exhibiting Th1 functional potential in BCG vaccinated (n = 8) and combo vaccinated (n = 8) heifers.

    Journal: Frontiers in Immunology

    Article Title: Effect of co-vaccination of cattle with RB51 and BCG on vaccine-specific CD4 + T cell responses

    doi: 10.3389/fimmu.2025.1664398

    Figure Lengend Snippet: Th1 functional potential of vaccine-specific CD4 + T cells in dairy-beef heifers. (A) Quadrant gate defines functional subsets of CD4 + T cells: Q1, proliferating and IFN-γ + ; Q2, IFN-γ + only; Q3, unresponsive; Q4, proliferating only. (B, C) Bar height represents total average number of responding CD4 + T cells; broken down into IFN-γ + only (white bars), proliferating only (gray bars), and proliferating and IFN-γ + (black bars) subsets. (B) Mean number of RB51-specific CD4 + T cells exhibiting Th1 functionality in RB51 vaccinated (n = 8) and combo vaccinated (n = 8) heifers. (C) Mean number of BCG-specific CD4 + T cells exhibiting Th1 functional potential in BCG vaccinated (n = 8) and combo vaccinated (n = 8) heifers.

    Article Snippet: Cells were then incubated with FITC labeled anti-bovine CD4 (clone CC8, Cat. No. MCA1653F, BioRad, Hercules, CA) antibody for 15 min at RT.

    Techniques: Functional Assay

    Proliferation of CD4 + T cells in response to in vitro PPDb stimulation in BCG and combo vaccinated Holstein steers. The average number of CD4 + T cells (gray bars) proliferating in response to PPDb stimulation from unvaccinated (white circles, n = 8), BCG vaccinated (blue circles, n = 7), and combo vaccinated (black circles, n = 8) Holstein steers. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. NS, no significance .

    Journal: Frontiers in Immunology

    Article Title: Effect of co-vaccination of cattle with RB51 and BCG on vaccine-specific CD4 + T cell responses

    doi: 10.3389/fimmu.2025.1664398

    Figure Lengend Snippet: Proliferation of CD4 + T cells in response to in vitro PPDb stimulation in BCG and combo vaccinated Holstein steers. The average number of CD4 + T cells (gray bars) proliferating in response to PPDb stimulation from unvaccinated (white circles, n = 8), BCG vaccinated (blue circles, n = 7), and combo vaccinated (black circles, n = 8) Holstein steers. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. NS, no significance .

    Article Snippet: Cells were then incubated with FITC labeled anti-bovine CD4 (clone CC8, Cat. No. MCA1653F, BioRad, Hercules, CA) antibody for 15 min at RT.

    Techniques: In Vitro